Publications

A rapid method for determining dynamic binding capacity of resins for the purification of proteins

Do T, Ho F, Heidecker B, Witte K, Chang L, Lerner L.  Protein Expr. Purif. 2008 August;60(2):147-50.

Biolayer interferometry is a novel method for quantifying macromolecules, such as proteins, in solution. The presence of other, non-binding molecules does not interfere with quantification, which allows one to measure the concentration of the molecule of interest in a crude mixture. Here we apply this method to determining the dynamic binding capacity of affinity resins.